Chemical and Biological Strategies for Profiling Protein‐Protein Interactions in Living Cells

Elucidation of Protein-Protein Interactions (PPIs) in living cells/animals is very important for the study basic biology and development novel therapeutics. Recent advances techniques profiling PPIs, such as fluorescence microscopy various proximity labeling-based methods coupled with mass spectrometry (MS), have provided a great opportunity to PPIs their native states spatiotemporal resolution, especially ones that are inaccessible conventional co-immunoprecipitation (co-IP) pull-down assays. More information can be found Review by You-Yu Wang, Wenyi Li, Bang-Ce Ye, Xiao-Bao Bi.